Neurofunctional behavior and recognition influence of rat bone marrow mesenchymal stem cells transplant from lateral cistern to cerebral vasospasm rat
10.3760/cma.j.issn.1674-6554.2013.06.004
- VernacularTitle:骨髓间质干细胞侧脑室植入对脑血管痉挛大鼠神经功能及认知功能的作用
- Author:
Guangyu HAO
;
Zhiping CAI
;
Dongsheng HUO
;
Gang FANG
;
Suqin SHI
;
Zhaohui LI
;
Ming ZHANG
- Publication Type:Journal Article
- Keywords:
Subarachnoid;
Cerebral vasospasm;
Neurofunctional score;
Morris maze
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2013;(6):491-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether bone marrow mesenchymal stem cell transplantation can improve vasospastic rats sense and motor function.Methods Rats grouped with randomized number method as Control group,Subarachnoid hemorrhage group.Stem cell culture media group and Stem cell transplantation group.Subarachnoid hemorrhage model were made with tail artery blood twice injection,2 days after 2' nd injection.Bone marrow mesenchymal stem cell were transplanted to lateral cistern.Subarachnoid hemorrhage(SAH) group didn' t transplant stem cell.Stem cell culture media group injected DMEM media as DMEM group.Stem cell transplantation group injected 30μl Bone Marrow mesenchymal stem cell suspension,so called BMSCs group.Neurofunctional score and learning memory expression were detected with morris mazer and Neurofunctional Score Scale in each group.Results After transplantation for 7 d,functional score of Control,SAH,DMEM and Stem cell group were 3.95 ±2.51,7.20 ± 1.03,7.23 ± 1.79 and 5.81 ± 1.11 respectively.Compared with others groups,Stem cell group score was significantly decrease(P=0.017).After transplanting stem cell for 14 d,the mean spanning plate time in Control group,SAH group,DMEM group and Stem cell group were 7.38 ± 1.73,4.52 ± 0.90,5.11 ± 1.93 and 7.32 ± 2.16 respectively,SAH and DMEM group vs other 2 groups,there were clearly statistically differences (P =0.009),while between control group and stem cell group,there were no statistically differences (P =0.14).Conclusion SAH rat transplant stem cell can improve sense,motor and learning expression in certain level.
