Comparison of the construct validity of anxiety animal models in Kunming mice between young and old
10.3760/cma.j.issn.1674-6554.2013.06.011
- VernacularTitle:青年与老龄昆明小鼠焦虑动物模型结构维度比较
- Author:
Lina WANG
;
Xiaobing GUO
;
Jinfang MA
;
Mingjun DU
;
Fei PAN
- Publication Type:Journal Article
- Keywords:
Age;
Anxiety;
Animal model;
Open field test;
Elevated plus maze
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2013;(6):514-516
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of different ages on behavioral dimensions of anxiety animal models in Kunming mice.Methods Young/Old,male,Kunming mice were recorded twice,for five minutes,with a one-week inter-trial interval in open field test (OFT),elevated plus maze (EPM) and light/dark box (LDB).The following parameters were evaluated:percentage of time spent in the OFT central area (Ctime%),percentage of number of squares visited in the OFT central area (Ccross%),the total number of horizontal squares in OFT (Cross),the total number of rears in OFT (Rear),percentage of time spent in the EPM open arms (Otime%),percentage of number of entries into the EPM open arms (Oentries%),the total number of entries into the EPM open and closed arms (Entries),percentage of time exploring in the LDB light area (Ltime%),percentage of number of squares crossing in the LDB light area (Lcross%),percentage of rears in the LDB light area (Lrear%),the total number of transition in LDB (Transition),the total number of horizontal squares in LDB (Cross) and the total number of rears in LDB (Rear).Results OFT-Ctime%,OFT-Ccross%,EPM-Otime%,EPM-Oentries%,LDB-Ltime%,LDB-Lcross% and LDB-Lrear% had significant loadings on anxiety factor; OFTCross,EPM-Entries,LDB-Transition and LDB-Cross loaded on locomotion-exploration factor.Old mice had higher anxiety emotion (Ctime%,t =3.12,P< 0.01; Ccross%,t =2.37,P< 0.01; Otime%,t =1.31,P< 0.05; Oentries%,t=1.57,P<0.05; Ltime%,t=4.15,P<0.01 ; Lcross%,t=3.28,P<0.01 ; Lrear%,t=2.73,P<0.01) and lower locomotion and exploratory activity (Cross,t =2.05,P < 0.01 ; Entries,t =2.09,P < 0.05 ;Transition,t=2.51,P<0.01; Cross,t=3.02,P<0.01),compared with Young.Conclusion Ages have no effect on structural dimensions of anxiety animal models.With the growth of ages,old mice have higher anxiety emotion but lower locomotion and exploratory activity.