Effects of levetiracetam on the expression of SYN and GAP-43mRNA in the hippocampus of rats with epilepsy
10.3969/j.issn.1000-3606.2013.07.019
- VernacularTitle:左乙拉西坦对癫大鼠海马组织中SYN和GAP-43 mRNA表达的影响
- Author:
Yan ZHANG
;
Haiyan WANG
;
Ying WANG
;
Lei WANG
;
Lei NIE
- Publication Type:Journal Article
- Keywords:
levetiracetam;
epilepsy;
synaptophysin;
growth-associated protein 43;
rat
- From:
Journal of Clinical Pediatrics
2013;(7):670-673
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To observe the effects of levetiracetam (LEV) on spatial learning and memory ability in epileptic rats and the expression of synaptophysin (SYN) and growth-associated protein 43 (GAP-43) in the hippocampus of rats with epi-lepsy induced by lithium pilocarpine (Li-pilo). Methods SD rats were randomly divided into normal control group (normal saline group), LEV group (treatment group), Li-pilo group (model group) and LEV control group (normal dose group). Twenty-four hours after the establishment of model, LEV (150 mg/kg per day) was given to rats in LEV group and LEV control group, and equivalent normal saline was given to rats in normal control group and Li-pilo group. At 7, 14 and 28 days after successful model building, the expression levels of the SYN and GAP-43mRNA in hippocampus of rats in each group were detected by RT-PCR. In the 4th week, the ability of learning and memory of rats in each group was evaluated by the Morris water maze. Results There was no significant difference in escape latency and the frequency of crossing platform between LEV control group and normal control group in Morris water maze test. Compared with normal control group, the rats in Li-pilo group had a longer latency to escape and reduced frequency of crossing platform (P<0.05). Compared with Li-pilo group, the rats in LEV group had shorter latency to escape and more frequency of crossing platform (P<0.05). There was no significant difference in the expressions of SYN and GAP-43mRNA in hippocampus of rats between LEV control group and normal control group. The expressions of SYN and GAP-43mRNA in Li-pilo group were lower than those of normal control group and LEV group (P<0.05). Conclusions Levetiracetam may improve learning and memory ability of rats with epilepsy through the regulation of SYN and GAP-43mRNA expressions in hippocampal tissue.
