Effect of shRNA silenced aromatic hydrocarbon receptor on WNT signaling pathway during the differentiation of cardiac myocytes

VernacularTitle:shRNA沉默芳香烃受体表达对心肌细胞分化过程中WNT信号通路的影响
Author: Zhigang FAN ; Chun ZHU ; Zhangbin YU ; Xiaoshan HU ; Mengmeng LI ; Jingai ZHU ; Shasha ZHU ; Shuping HAN
Publication Type:Journal Article
Keywords: aromatic hydrocarbon receptor; differentiation of cardiac myocytes; WNT signaling pathway; mouse
From: Journal of Clinical Pediatrics 2013;(7):666-669
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of shRNA silenced aromatic hydrocarbon receptor (AHR) on WNT signaling path-way during the differentiation of P19 cells into cardiac myocytes. Methods The eukaryotic expression vector of mouse AHR gene was designed and constructed. The interference plasmid was transfected into P19 cell and the positive stains to AHR gene silencing were screened by G418. The mRNA expression of important genes GSK3βandβ-catenin were evaluated by real-time fluorescent quantita-tive PCR during the differentiation of P19 cells. Results The constructed AHR-shRNA plasmid significantly inhibited the expression of AHR gene. Along with the differentiation of P19 cell into cardiac myocytes, in the interference group the expression ofβ-catenin gene was lower whereas the expression of GSK3βgene was elevated than those of control group with significant differences (all P<0.01). Conclusions The interference of AHR gene expression can regulate WNT signaling pathway in the development of heart.