Expression of annexin A1 in non-small cell lung cancer tissues and its significance
10.3969/j.issn.1000-8179.2013.15.007
- VernacularTitle:膜联蛋白A1在非小细胞肺癌组织中的表达及意义*
- Author:
Hongling LI
;
Tao SHEN
;
Yalian SA
;
Xinmin YAN
- Publication Type:Journal Article
- Keywords:
non-small cell lung carcinoma;
annexin A1;
gene expression
- From:
Chinese Journal of Clinical Oncology
2013;(15):907-910
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to detect the expression of annexin A1 in human non-small cell lung cancer (NSCLC) and to explore its clinical significance. Methods:We detected the expression levels of annexin A1 in NSCLC and the same patient's dis-tal cancerous tissue (from the edge of the tumor >5 cm) by real-time fluorescence quantitative polymerase chain reaction (real-time PCR), Western blot, and immunohistochemical methods, and then analyzed their correlation with clinical pathological parameters. Re-sults: Real-time PCR showed that the expression of annexin A1 mRNA in NSCLC was higher than that of distal cancerous tissue (0.574±1.403 vs. 0.240±0.893, t=2.060, P=0.045). Moreover, the expression of annexin A1 in NSCLC was associated with the degree of differentiation, lymph node metastasis, and TNM staging (P<0.05), but independent of gender, age, smoking history, tumor size, and histological type (P>0.05). Western blot and immunohistochemistry revealed that the expression of annexin A1 protein in NSCLC was higher than that of distal cancerous tissue. Conclusion:Annexin A1 is highly expressed in the cancer tissues of patients with NSCLC, which may be correlated with the occurrence and development of tumor and its invasion and metastasis.
