Induction ways of bone marrow mesenchymal stem cells differentiating into nerve cells★
10.3969/j.issn.2095-4344.2013.32.003
- VernacularTitle:诱导剂共培养：谁更适宜骨髓间充质干细胞向神经细胞的分化？★
- Author:
Zengsheng CHEN
;
Qiang CHU
;
Yanfeng LIU
;
Xuan SONG
;
Ping LI
- Publication Type:Journal Article
- Keywords:
stem cel s;
bone marrow stem cel s;
bone marrow mesenchymal stem cel s;
nerve cel s;
induced differentiation;
chemical induction;
co-culture;
neuron-specific enolase;
stem cel photographs-containing paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(32):5757-5764
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Currently, bone marrow mesenchymal stem cel s can differentiate into nerve cel s via many approaches. Different methods for inducing bone marrow mesenchymal stem cel s differentiating into nerve cel s have different ratios. OBJECTIVE:To investigate the difference between chemical method and co-culture method to induce the differentiation of rat bone marrow mesenchymal stem cel s into nerve cel s. METHODS:Rat bone marrow mesenchymal stem cel s were isolated and purified using whole bone marrow culture method, and then randomly divided into two groups:chemical group,β-mercaptoethanol was added;co-culture group, co-cultured in a Transwel chamber. RESULTS AND CONCLUSION:Visible protrusions from induced cel s showed radiation growth at 1 week of induced culture, and neuron-specific enolase staining was positive at 2 weeks of culture. Star-like structure of nerve cel s was visible in the co-culture group within 4-5 days of culture, and then more protrusions formed. Meanwhile, the positive rate of neuron-specific enolase was (70.82±2.46)%. After 6-7 days of culture, neuron-like cel s formed and were interconnected in the chemical group;while, the positive rate of neuron-specific enolase was (52.37±1.83)%. These findings suggest that cel microenvironment plays a leading role in the differentiation of bone marrow mesenchymal stem cel s into nerve cel s, and chemical induction method is inferior to the co-culture method.
