Oxidative stress effect on viability of umbilical cord-derived mesenchymal stem cells in storage solution of transplantation
10.3969/j.issn.2095-4344.2013.32.007
- VernacularTitle:移植保存液中脐带间充质干细胞活性与氧化应激的影响★
- Author:
Yuhu NIU
;
Yan CHEN
;
Jianlin ZHANG
;
Xin LEI
;
Yanting DONG
;
Lei CUI
;
Bo NIU
- Publication Type:Journal Article
- Keywords:
stem cel s;
umbilical cord/umbilical blood stem cel s;
human umbilical cord-derived mesenchymal stem cel s;
oxidative stress;
active oxygen;
senescence;
glutathione;
malondialdehyde;
superoxide dismutase;
stress level;
stem cel photographs-containing paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(32):5785-5792
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The viability of human umbilical cord-derived mesenchymal stem cel s is often declined with the commonly used transplantation storage solution in clinics, which may influence the therapeutic effects of cel ular transplantation. However, reasons for this are stil unknown. OBJECTIVE:To investigate the role of oxidative stress in the reduction of human umbilical cord-derived mesenchymal stem cel s viability in the storage process during clinical transplantation and to observe the effects of radical scavenger on the results. METHODS:Human umbilical cord-derived mesenchymal stem cel s were harvested and cultured in normal saline for 0, 2, 4 and 6 hours at room temperature. Intracel ular reactive oxygen levels were detected at those time points. Antioxidant enzyme activities and levels of malondialdehyde were measured to determine the intracel ular oxidative stress levels after storage. Cel adhesion rate changes were retested after adding N-acetyl cysteine to the storage solution. RESULTS AND CONCLUSION:The reactive oxygen levels in human umbilical cord-derived mesenchymal stem cel s were increased significantly after normal saline storage and levels of malondialdehyde were increased in a time-dependent manner. Activities of superoxide dismutase, catalase and glutathione peroxidase were al reduced. Addition of N-acetyl cysteine into the storage medium decreased the reactive oxygen levels and improved the human umbilical cord-derived mesenchymal stem cel s viabilities. Experimental findings indicate that, increased reactive oxygen species in human umbilical cord-derived mesenchymal stem cel s is one of the reasons for reduced cel viability. Adding the radical scavenger N-acetyl cysteine can improve the storage effects of human umbilical cord-derived mesenchymal stem cel s.
