Three-dimensional structure and morphology of the mandible in type 1 diabetes mellitus mice
10.3969/j.issn.2095-4344.2013.28.001
- VernacularTitle:1型糖尿病小鼠下颌骨三维结构及组织形态*
- Author:
Jun ZHANG
;
Liting JIANG
;
Jinshen WANG
;
Qi ZHOU
;
Yiming GAO
- Publication Type:Journal Article
- Keywords:
tissue construction;
bone tissue construction;
type 1 diabetes mel itus;
mandibular bone structure;
cancel ous bone;
cortical bone;
bone microstructure;
bone morphometry;
micro-CT;
histological section;
provincial grants-supported paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(28):5101-5107
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Diabetes mel itus is one of the most common systemic diseases, which often leads to the changes of the jaw and other bone structure, as wel as the abnormal changes of mineral metabolism. OBJECTIVE: To observe the three-dimensional structure and histopathological changes of the mandible in type 1 diabetes mel itus mice. METHODS: The mice were randomly divided into control group and diabetes mel itus group. The diabetes mel itus group received intraperitoneal injection of 50 mg/kg streptozotocin for 5 days to establish a type 1 diabetes mel itus model, and the control group received intraperitoneal injection of citrate buffer. RESULTS AND CONCLUSION: At 3 weeks after modeling, the micro-CT technique was used to observe the three-dimensional structure of the mandibles in the two groups. The quantitative analysis on the microstructure of cancel ous bone and cortical bone showed that the bone mineral density, bone volume fraction, trabecular number and trabecular thickness of cancel ous bone in the interest region in the mandible of type 1 diabetes mel itus mice were significantly decreased when compared with that in the control group (P < 0.01, P < 0.05), while the structure model index was increased significantly (P < 0.05); the mineral density and area of cortical bone were decreased in the diabetes mel itus group (P < 0.05). Hematoxylin-eosin staining showed that the number and volume of mandibular trabeculae of type 1 diabetes mel itus mice were decreased. The results suggest that the three-dimensional structure of the cancel ous bone and cortical bone in the streptozotocin-induced type 1 diabetes mel itus mice are changed significantly, and the microstructure change of the cancel ous bone is more obvious.
