Ectopic bone formation in adipose-derived mesenchymal stem cell-seeded osteoinductive calcium phosphate scaffolds
10.3969/j.issn.2095-4344.2013.29.001
- VernacularTitle:脂肪间充质干细胞复合骨诱导性磷酸钙陶瓷支架的异位成骨☆
- Author:
Jinfeng YAO
;
Xiaowei ZHANG
;
Qi ZHOU
;
Cangshang ZHENG
;
Zhigang LIANG
;
Chongyun BAO
- Publication Type:Journal Article
- Keywords:
biomaterials;
tissue-engineered bone materials;
bone tissue engineering;
adipose-derived mesenchymal stem cells;
osteoinductive biphasic calcium phosphate ceramic;
ectopic bone formation;
Micro-CT
- From:
Chinese Journal of Tissue Engineering Research
2013;(29):5261-5268
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The phenomenon of osteoinduction by biomaterials has been proven in animal experiments. OBJECTIVE:To investigate whether the ability of a biomaterial to initiate bone formation in ectopic implantation sites improves the performance of osteoinductive biomaterial as a scaffold for tissue-engineered bone. METHODS:We compared ectopic bone formation by combining autologous adipose-derived stromal cells with an osteoinductive and a nonosteoinductive biphasic calcium phosphate ceramic to create a tissue engineering construction in the muscle of dogs. Al implants were implanted in the back muscle of 10 adult dogs for 8 weeks and 12 weeks, including osteoinductive biphasic calcium phosphate ceramic+adipose-derived stromal cells (osteoinductive complex group), osteoinductive biphasic calcium phosphate ceramic (osteoinductive broup), nonosteoinductive biphasic calcium phosphate ceramic+adipose-derived stromal cells (nonosteoinductive complex group), and nonosteoinductive biphasic calcium phosphate ceramic (nonosteoinductive group). Micro-CT analysis and histomorphometry were performed to evaluate and quantify ectopic bone formation. RESULTS AND CONCLUSION:Ectopic bone formation was visible in the osteoinductive complex group and osteoinductive group, and the former group was superior to the latter one in quality of new bone (P<0.05). However, there was no ectopic bone formation in the other two groups. Micro-CT results were consistent with the histomorphological detection. These findings indicate that osteoinductive biphasic calcium phosphate ceramic, as a kind of bone tissue engineering scaffold material, has a better osteogenic capacity, while adipose-derived mesenchymal stem cells serve as seed cells to promote the ectopic bone formation.
