Periodontal ligament cells from miniature swine grow on a hydroxyapatite scaffold
10.3969/j.issn.2095-4344.2013.29.005
- VernacularTitle:小型猪牙周膜干细胞在羟基磷灰石支架材料上的生长*★
- Author:
Luping MA
;
Liangjun ZHONG
;
Yuanming ZHANG
;
Yuan ZHANG
;
Pengtao ZHANG
- Publication Type:Journal Article
- Keywords:
biomaterials;
tissue-engineered oral materials;
periodontal ligament cells;
in vitro culture;
hydroxyapatite;
scanning electron microscope;
miniature swine;
provincial grants-supported paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(29):5290-5295
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Periodontal tissue engineering technology provides new ideas and new ways for periodontitis-induced bone defect repair. OBJECTIVE:To develop a culture model for the periodontal ligament cells of miniature swine, which was constructed with hydroxyapatite, to investigate the biocompatibility with hydroxyapatite. METHODS:Periodontal ligament cells from miniature swine were harvested by using tissue explant method. Immunofluorescence was used to detect the expression of stromal cel antigen 1 in the periodontal ligament cells of miniature swine. The third passage cells were co-cultured with a three-dimension hydroxyapatite scaffold, and the biological characteristics of the cells were observed under a scanning electron microscope at days 1, 3, 7 of co-culture. RESULTS AND CONCLUSION:The pirmary miniature swine periodontal ligament cells grew wel , and they were positive for stromal cel antigen 1. Under the scanning electron microscope, the periodontal ligament cells of miniature swine grew wel on the hydroxyapatite scaffold at days 1, 3, 7 of co-culture. These prove that the miniature swine periodontal ligament cells, which can be separated using tissue explant method and cultured successful y in vitro, can grow wel on the hydroxyapatite scaffold.
