Culture and identification of human embryo-derived myoblasts
10.3969/j.issn.2095-4344.2013.32.010
- VernacularTitle:人类胚胎来源成肌细胞的培养和鉴定*****★
- Author:
Guiying LIU
;
Liye YANG
;
Wenyu LI
;
Jiakun ZHENG
;
Qiang CHEN
- Publication Type:Journal Article
- Keywords:
stem cel s;
embryonic stem cel s;
myoblasts;
embryo;
myotube;
differentiation;
cel culture;
glial fibril ary acidic protein;
neuron specific enolase;
provincial grants-supported paper;
stem cel photographs-containing paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(32):5806-5812
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:There are myoblasts in human embryonic skeletal muscle. It remains poorly understand whether myoblasts in vitro can form myotube and what are the corresponding markers for identifying myoblasts and myotubes. OBJECTIVE:To investigate whether in vitro cultured myoblasts from human embryonic skeletal muscle can form myotube and whether they can express neural markers. METHODS:Human embryonic muscle-derived myoblasts were cultured in serum-containing medium. When the primary culture was established, cultured cel s were identified with immunocytochemistry for neural markers, such asβ-tubulin markers (desmin, myogenin, smooth muscle actin and myosin). RESULTS AND CONCLUSION:A population of myoblasts could migrate from human embryonic muscle tissues. They could express the markers for skeletal muscle such as desmin and myogenin, and they could express neuron specific enolase, nestin and neurofilament 200. They could form myotubes in vitro, and myotubes expressedβⅢ-tubulin, neurofilament 200 and glial fibril ary acidic protein. The data support the hypothesis that myoblasts from human embryonic muscle express neural markers and muscle markers, and cultured myoblasts and myotubes expressed neuron specific enolase,β-tubulin Ⅲ, nestin, neurofilament 200 and glial fibrillary acidic protein. This indicates that these markers could not be used for cel identification of trans-differentiation study from muscle origin to nervous system.
