Primary culture of umbilical cord-derived mesenchymal stem cells in serum free medium
10.3969/j.issn.2095-4344.2013.27.006
- VernacularTitle:无血清培养体系原代培养脐带间充质干细胞
- Author:
Tingting ZHOU
;
Chao WEI
;
Xiaodong CHEN
;
Hai LI
;
Zheng WANG
- Publication Type:Journal Article
- Keywords:
stem cells;
umbilical cord blood-derived stem cells;
umbilical cord-derived mesenchymal stem cells;
primary culture;
serum free;
serum containing;
replacing;
colony forming unit;
National Natural Science Foundation of China;
stem cell photographs-containing paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(27):4980-4987
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: The application of mesenchymal stem cells derived from umbilical cord cultured in serum-containing medium has some obstacles. OBJECTIVE: To establish serum free medium system for primary culture of umbilical cord-derived mesenchymal stem cells. METHODS: Wharton’s Jel y was isolated from umbilical cord, minced, 1-3 mm3, and subsequently incubated in either serum containing medium or serum free medium. Some cells were harvested on days 11, 14 and 17 for some detection. Based on the minimal criteria established by the International Society for Cel ular Therapy in 2006, mesenchymal stem cells were assayed with colony forming unit-fibroblast. RESULTS AND CONCLUSION: The mesenchymal stem cells grew rapidly in serum free medium condition compared with serum containing medium. On day 11, the number of colonies was larger in serum free medium condition than that in serum containing medium. Thus, serum free medium could maintain properties of mesenchymal stem cells and provide possibility a credible alternative to serum containing medium for primary culture of umbilical cord-derived mesenchymal stem cells.
