Telomere-associated factor expression in replicative senescence of human embryonic lung fibroblasts
10.3969/j.issn.2095-4344.2013.28.014
- VernacularTitle:人胚肺成纤维细胞复制性衰老过程中端粒相关因子的表达*★
- Author:
Hua DU
;
Xiaoyan XU
;
Ling HAI
;
Yingxu SHI
- Publication Type:Journal Article
- Keywords:
tissue construction;
tissue construction and bioactive factors;
replicative senescence;
telomere binding protein 1;
Tankyrase 1;
telomere protection protein 1;
telomerase RNA;
P53;
other grants-supported paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(28):5184-5190
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Telomere-associated proteins wil directly affect the function of telomeres, adjust the length of telomeric DNA, which are closely related with cellsenescence and carcinogenesis. OBJECTIVE: To find the key regulatory molecules in the cellsenescence process through observing the telomere-associated factor expression in normal cel replicative senescence process. METHODS: Based on established cel replicative senescence model, reverse transcription-PCR and western blot were used to detect the telomere-associated factor expression on the molecular and protein levels, including the telomere-associated factor human telomere binding protein 1, tankyrase 1, telomerase RNA, telomere protection protein 1 and P53 expressions in the human embryonic lung fibroblast replicative senescence. RESULTS AND CONCLUSION: The results showed that with the cellsenescence, transcription of human telomere binding protein 1 did not changed, while the protein expression of human telomere binding protein 1 was increased gradual y and then decreased rapidly; mRNA and protein expressions of telomere protection protein 1 did not changed; with the human embryonic lung fibroblast replicative senescence, expression of telomere protection protein 1 was decreased gradual y; with cellsenescence, telomerase RNA component showed an increasing trend; protein expression of P53 did not changed. Human telomere binding protein 1, telomere protection protein 1 and telomerase RNA play an important role in cellsenescence.
