Study of the effect of JNK signal transduction pathway in intense noise-induced apoptosis in cochlea of guinea pig
10.3969/j.issn.1001-1781.2009.24.010
- VernacularTitle:JNK信号通道在强噪声诱导豚鼠耳蜗细胞凋亡的作用
- Author:
Qiuhong XUE
;
Jia CHEN
;
Shusheng GONG
;
Jing XIE
;
Jian HE
;
Xiaolin CHEN
- Publication Type:Journal Article
- Keywords:
intense noise;
apoptosis;
JNK
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2009;(24):1138-1142
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of intense noise-induced cochlea cells death in guinea pig,and the effect of JNK signal transduction pathway in the procedure of cochlea cells apoptosis by intense noise-induced.Method:Thirty-two guinea pigs were randomly divided into 4 groups.The guinea pigs in the experiment groups were exposed to 4 kHz narrow band noise at 120 dB SPL for 4 h.After the noise expose for 1,4,14 days of the experiment guinea pigs,ABR of the guinea pigs on experiment and control groups were tested before put them to death.Four guinea pig's cochleas of every group were taken to paraffin section,and the rest was extracted the total cochlear's protein.Apoptosis was tested by terminal deoxynucleotidyl Transferase(TdT)-mediated deoxyuridine triphosphate(d-UTP)nick and labeling method(TUNEL).The phosphorylation of JNK and c-Jun were tested by immunohistochemistry and western blot methods.Result:Tunel-Positve cells in the Corti's,SGC and SV of experiment groups,and there have significant differences compared with the control group(P＜0.01)and Tunel-Positve cells are most in 1 d experiment group.The positive cells of P-JNK and p-e-Jun could be dectected in guinea pig's cochleas after noise exposed,but no positive cells were found in the control.Protein levels of p-JNK,and P-c-Jun were risen up and activated quickly after noise exposed,and achieved peak in 1 d,4 d,and then fallen-offs,but still maintained higher levels within 14 d.Conclusion:Intense noise causes cochlea cell lesion by inducing apoptosis to result in and JNK signal transduction pathway plays an important role in the procedure of apoptosis.
