Effect of anti-HER-2 engineered antibody chA21 on angiogenesis in nude mice xenografts of human ovarian cancer SKOV3 cells

VernacularTitle:抗HER-2工程抗体chA21对人卵巢癌SKOV3裸小鼠移植瘤血管生成的影响
Author: Anli ZHANG ; Qiang WU ; Feng YANG ; Guodong SHEN ; Jing LIU
Publication Type:Journal Article
Keywords: ovary neoplasms; HER-2; chA21; VEGF; DLL4; angiogenesis
From: Chinese Journal of Clinical and Experimental Pathology 2009;(6):627-630
CountryChina
Language:Chinese
Abstract: Purpose To investigate the effect of anti-HER-2 engineered antibody chA21 on the angiogenesis in nude mice xenografts of human ovarian cancer SKOV3 cells that overexpresses HER-2.Methods An animal model with SKOV3 cells involved in nude mice was established and the mice were randomized into control group and chA21 group;chA21 was administrated via caudal vein for 5 weeks, meanwhile the same volume of chA21 buffer was injected in control group.The mice were terminated after 5 weeks and the xenografts dislodged,VEGF and DLL4 expression in two groups were quantitatively analyzed by immunohistochemistry on tissue microarray sections combined with a micro-image analyzing system, and microvessel density (MVD) values were compared between the two groups by immunohistochemical staining of CD31.Results The expression of VEGF, DLL4 and MVD values in chA21 group were lower than those in control group (P<0.05).Conclusion chA21 inhibits the angiogenesis of transplantations of human ovarian cancer SKOV3 cells effectively.