Research on multiplication of the H9N2 subtype avian influenza virus in large-scale microcarrier-based MDCK cell culture system
10.3969/j.issn.1002-2694.2009.12.005
- VernacularTitle:微载体规模化培养MDCK细胞增殖H9N2亚型禽流感病毒的研究
- Author:
Chunyan LI
;
Jing XIAO
;
Xi LI
;
Di LIU
- Publication Type:Journal Article
- Keywords:
microcarriers;
H9N2 subtype avian influenza;
Madin-Darbin canine kidney (MDCK) cell;
large-scale culture
- From:
Chinese Journal of Zoonoses
2009;(12):1149-1153
- CountryChina
- Language:Chinese
-
Abstract:
To explore the regularity for the multiplication of avian influenza virus subtype H9N2 in large-scale microcarrier-based MDCK cell culture system, and to determine the optimal proliferation conditions. H9N2 subtype of avian influenza virus was inoculated into the MDCK cell growing on 24 well plate, and the HA titers of virus at different time were detected in the conditions of different infectious doses,different concentrations of TPCK- trypsin and different pH. The optimal conditions were determined. Then the H9N2 subtype avian influenza virus was grown in microcarrier-based MDCK cell in 250mL and 5L roller bottles. It was demonstrated that high viruse yield with a hemagglutination unit of 9 log2(1:512) could be obtained under the optimal conditions of multiplication . The result indicated the H9N2 subtype avian influenza virus could be produced in microcarrier-based MDCK cell in a large-scale culture system with a high virus yield and demonstrates the feasibility of the development of mammalian cell-based in influenza vaccine in microcarrier culture systems.
