Development of the mouse model for acute hepatitis B virus infection
10.3969/j.issn.1002-2694.2009.12.010
- VernacularTitle:急性乙型肝炎病毒感染小鼠模型的建立
- Author:
Chunxia GUO
;
Yongwen HE
;
Cheng PENG
;
Wenting LI
;
Zhihong WENG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
hydro-dynamics based transfection;
animal model
- From:
Chinese Journal of Zoonoses
2009;(12):1170-1173
- CountryChina
- Language:Chinese
-
Abstract:
A mouse model for acute hepatitis B virus (HBV) infection was established by using the hydrodynamical injection of mouse tail vein, in which the immunocompetent BALB/c mice were hydrodynamically injected with a competent replication plasmid pAAV-HBV1.2 having 1.2 fold over-length of HBV DNA. On day 1, 2, 4, 6 and 8 after injection, the levels of HBsAg, HBeAg and HBV DNA in blood serum were detected by using ELISA and fluorogenic quantitative PCR assay (FQ-PCR). And on day 8. HBsAg and HBeAg in liver tissue were assayed by immunohistochemical staining. It was found that HBsAg in blood serum could be detected on day 1 after infection in 14 of 16 mice (85.7%) injected with pAVV-HBV1.2 by using ELISA assay and the peak levels of HBsAg and HBeAg were attained during the first day after injection and then it dropped down gradually up to day 8 following injection. The titer of HBV DNA in blood serum attained its peak on day 2 and maintained a high level later on. On day 8 after injection, its titer was 1.9×10~4 copies/mL. The percentage of HBcAg-positive hepatocytes and HBsAg-positive hepatocytes in liver tissues were 5% and 2% respectively. Thus, by using the hydrodynamic injection with the competent replication plasmid, a mouse model for acute HBV infection is successively developed.
