The inhibitory effect of gadolinium chloride on the regulatory T cells of mice with hepatic granuloma caused by Schistosoma japonicum
10.3969/j.issn.1002-2694.2009.12.011
- VernacularTitle:氯化钆对血吸虫肉芽肿期肝脏调节性T细胞的抑制作用
- Author:
Jianghua YANG
;
Lei HE
;
Tao WANG
;
Jinsun YANG
;
Weishun HOU
;
Chuan SU
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
CD4~+CD25~+T cells;
Gadolinium chloride;
Kupffer cells
- From:
Chinese Journal of Zoonoses
2009;(12):1174-1176
- CountryChina
- Language:Chinese
-
Abstract:
To determine whether the targeting inhibition of Kupffer cellsfunction mediated by gadolinium chloride (GdCl_3) could interfere with the CD4~+CD25~+ regulatory T cells of mice at the granuloma stage of schistsomiasis, female C57BL/6 mice of 6-8 weeks old were divided randomly into 3 groups, i.e. control group. group infected with cercariae of Schistsoma japonicum and group of infection plus GdCl3,. GdCl3 in a dosage of 15 mg/kg was introduced into mice through penile vein twice per week. The number of CD4~+CD25~+ T cells was determined using flow cytometry and the number of cells with Fox p3 was detected by using immunohistochemical methods. For detection of cytokines, such as IL-4, IL-5, IL-10, TGF-β1, ,IFN-γ in mouse sera, a DuoSer ELISA development kit was used, It was found that the number of CD4~+CD25~+ T cells and level of IL-10 in Schistosomiasis granuloma stage were decreased in the S.japonicum cercariae infected mice injected with GdCl_3 in comparison with the infection group. The percentages of CD4~+CD25~+ T cells of infection group and infection plus GdCl_3 group were 13.8%, 9.3% and 6.4% respectively, while the levels of IL-10 of these 3 groups of rats were 41.4 pg/mL, 22.6 pg/mL and 11.5% respectively. In addition, treatment with GdCl_3 could down-regulate the expression of Fox p3 and reduce the inflammatory reactions in Schistosomiasis granuloma. It is evident that the targeting inhibition of Kupffer cellsfunction mediated by GdCI_3 interfere with the production of the regulatory T cells and reduce the inflammatory responses in Schistsomiasis granuloma.
