Parallel detection of the circulating antigens and antibodies in the diagnosis of schistosomiasis
10.3969/j.issn.1002-2694.2009.12.022
- VernacularTitle:循环抗体与循环抗原组合检测诊断血吸虫病的应用观察
- Author:
Weiming LAN
;
Honggen CHEN
;
Xiaojun ZENG
;
Weisheng JIANG
;
Jun GE
;
Shuying XIE
- Publication Type:Journal Article
- Keywords:
schistosomiasis;
circulating antigen;
circulating antibody;
united test;
ELISA
- From:
Chinese Journal of Zoonoses
2009;(12):1218-1221
- CountryChina
- Language:Chinese
-
Abstract:
To determine the diagnostic efficiency of parallel detection of the circulating antigens and antibodies in schistosomiasis, sandwich ELISA and indirect ELISA by using the labeled McAb JPG3 were used to detect the presence of the circulating antigens and the circulating IgG antibodies in serum samples from different kinds of population. and then the sensitivity and specificity of this method of testing as well as the efficiency of the application of this method in heavy endemic area. were determined in comparison with serial test. It was found that the sensitivity and specificity of the parallel test were 97.9% and 92.2% , however, those of the serial test were 76.0% and 99.2% respectively. The positive rates of parallel test and serial test to detect the stool examination-positive for schistosoma eggs in population of the endemic area were 94.6% (35/37) and 67.6% (25/37), while those to detect the stool examination-negative for schitosoma eggs were 69.8% (97/139) and 39.6% (55/139) respectively. It is apparent the parallel test for the detection circulating antigens and antibodies in schistosomiasis shows its high diagnostic efficiency, especially in the heavy endemic area of schitosomiasis.
