Study on the protective function and its mechanism of cyclosporin A to immature brain tissue with convulsive brain damage
10.3969/j.issn.1000-3606.2009.11.008
- VernacularTitle:环孢素A对未成熟脑惊厥性损伤的保护作用及机制研究
- Author:
Yale GUO
;
Shaoping HUANG
;
Dan LI
;
Lin YANG
;
Jianping ZHOU
- Publication Type:Journal Article
- Keywords:
eyclosporin A;
epilepsy;
immature brain;
glial fibrillary acidic protein;
P-glycoprotein
- From:
Journal of Clinical Pediatrics
2009;(11):1030-1035
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective function and its mechanisms of eyclosporin A to immature brain tissue with convulsive brain damage. Methods 21-day-old SD rats were given lithium-pilocarpine to make the epilepsy model. Total 67 male rats had been investigated. Cyclosporin A (CsA) were injected three times at 6, 30, 54 hrs after model had been established. Three dosages had been chosen: 5, 10 and 25 mg/kg each time. The level of apoptotic cells, P-glycoprotein (P-gp), glial fibrillary acidic protein (GFAP) in CA1 area of hippocampus had been determined, and compared with the rats without giving CsA. Results Rats from epilepsy model group had higher level of apoptosis, P-gp, GFAP expression than those from pseudo-model group. CsA injection by dose 5 mg/kg each time for three times reduced the level of P-gp, GFAP. Model group and pseudo-model group were same. Both the interventions of CsA injection by 10 mg/kg and 25 mg/kg can reduce the level of P-gp, GFAP, however neither of their effectiveness was better than CsA 5 mg/kg each time. Conclusions Small dosage of CsA may protect the immature brain tissue from convulsive brain damage by reducing the level of P-gp, GFAP in CA1 area of hippocampus.
