Role and mechanism of Astragalus in alleviating adhesion of neutrophils to human renal tubular cells induced by postasphyxial-serum of neonate
10.3969/j.issn.1000-3606.2009.11.019
- VernacularTitle:黄芪减轻窒息后血清诱导HK-2细胞黏附中性粒细胞的作用及机制
- Author:
Hongyu YANG
;
Wenbin DONG
;
Qingping LI
;
Xiaoping LEI
;
Tao XIONG
;
Cunliang DENG
;
Feng CHEN
- Publication Type:Journal Article
- Keywords:
Astragalus;
asphyxia;
renal tubular epithelial cell;
myeloperoxidase;
intercellular adhesion molecule-1
- From:
Journal of Clinical Pediatrics
2009;(11):1070-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Astragahs in alleviating adhesion of neutrophils to HK-2 cells induced by postasphyxial-serum of neonate and its signal transduction mechanism. MethodsHK-2 cells were used as target cell. Control group, asphyxia group, Astragalus group were divided in the experiment. The 20％ (volume fraction) postasphyxial-serum was used as attacking factor. The following indicators were detected: cellular morphology and neutrophils adhesion were observed with inverted microscope. The activity of myeloperoxidase (MPO) in the cell suspension were determined by biochemistry assay as the indicator for adhesion of neutrophils to HK-2. Intercellular adhesion molecule-1(ICAM-1) were examined by flow eytometer. ResultsThe neutrophils numbers (MPO activity) and ICAM-1 expression in asphyxia group were higher than that of the control group, but dramatically decreased in Astragalus group (P < 0.05). ConclusionsThese data demonstrated that Astragalus could alleviate the adhesion of neutrophils from neonate with asphyxia to HK-2 and it' s intracellular signal transduction mechanism is presumably involved in the inhibition of ICAM-1 expression on HK-2 cellular membrane.
