Effect of glucocorticoids on expression of aquaporin-1 in rats with acute lung injury
10.3760/cma.j.issn.1673-4912.2013.03.009
- VernacularTitle:糖皮质激素对急性肺损伤幼鼠水通道蛋白-1表达的作用
- Author:
Le MA
;
Zhengjuan LIU
;
Xuemei BAI
;
Qing WANG
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Lipopolysaccharide;
Aquaporin-1;
Glucocorticoids;
Rat
- From:
Chinese Pediatric Emergency Medicine
2013;20(3):265-267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of glucocorticoids in the treatment of acute lung injury(ALI) by studying the expression changes of aquaporin-1 (AQP-1) in the lung.Methods SpragueDawley rats were randomly divided into 3 groups,which were ALI group injected by lipopolysaccharide (LPS group),therapy group treated by methylprednisolone (Mp) after LPS injection (Mp group) and control group injected by normal saline.PaO2 and wet/dry mass ratio were assayed at 6 hours after the injection.Changes of AQP-1 expression in the lungs were analyzed by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR),and pathological examination was performed in each group.Results RT-PCR results showed that the expression of AQP-1 in LPS group was lower than that in control group (0.28 ± 0.05 vs 0.50 ± 0.24,P ＜ 0.05),while the expression of AQP-1 in Mp group was higher than that in LPS group (0.47 ±0.16 vs 0.28 ±0.05,P ＜0.05).The immunohistochemistry results were consistent to the RT-PCR results,meanwhile glucocorticoids therapy could improve the blood oxygen level (P ＜ 0.01),reduce the wet/dry mass ratio (P ＜ 0.05) and relieve the lung injury.Conclusion Glucocorticoids may have beneficial effects on ALI by affecting the expression of AQP-1 in the lungs of rats.
