The levels of interferon-γ, interleukin-4 and interleukin-5 in bronchoalveolar lavage fluid of BALB/c mice infected by Mycoplasma pneumonia
10.3760/cma.j.issn.1673-4912.2013.03.008
- VernacularTitle:肺炎支原体感染对BALB/c小鼠肺泡灌洗液中干扰素-γ、白细胞介素-4及白细胞介素-5含量的影响
- Author:
Xiang LI
;
Han ZHANG
;
Yunxiao SHANG
- Publication Type:Journal Article
- Keywords:
Mycoplasma pneumonia;
Asthma;
Bronchoalveolar lavage fluid;
Interferon-γ;
Interleukin-4;
Interleuin-5;
Mice
- From:
Chinese Pediatric Emergency Medicine
2013;20(3):261-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Mycoplasma pneumonia (MP) on the levels of interferon (IFN)-γ,interleukin (IL)-4 and IL-5 in bronchoalveolar lavage fluid (BALF) of BALB/c mice infected by MP,and to study whether MP infection may be involved in the pathogenesis of asthma through the pathway of influencing T helper type 1 (Th1)/T helper type 2 (Th2) immune response.Methods Forty BALB/c mice were randomly divided into two groups,control group and MP infected group,each group 20 mice.The BALF of MP infected group was respectively collected after MP infection at 3,7,14,21 days,each time 5 mice.The levels of IFN-γ,IL-4 and IL-5 were detected by enzyme linked immunosorbent assay.Results The levels of IFN-γ,IL-4 and IL-5 increased in MP infected group compared with control group,the differences were significant respectively (P ＜ 0.05).The levels of IFN-γ,IL-4 and IL-5 reached the peak at 7 d after MP infection.The IFN-γ/IL-4 ratio was significantly lower in MP infected group than control group,and achieved the trough at 7 d,the difference was significant (P ＜ 0.05).Conclusion MP infection can lead to the increased level of IFN-γ,IL-4 and IL-5 in BALF of BALB/c mice,peaked at 7 d.MP infection can increase the discharging of Th1 cytokines and Th2 cytokines,induce Th1/Th2 immune response disequilibrium,and it may be involved in the pathogenesis of asthmatic airway inflamation through the pathway.
