Mouse embryonic hepatic stem cells differentiate into cardiomyocyte-like cells
10.3969/j.issn.2095-4344.2013.32.011
- VernacularTitle:诱导小鼠胎肝干细胞向类心肌细胞的分化★
- Author:
Bigang WU
;
Jing CHANG
;
Xiaogang ZHANG
- Publication Type:Journal Article
- Keywords:
stem cel s;
embryonic stem cel s;
embryonic hepatic stem cel;
dimethylsulfoxide;
5-azacytidine;
induction;
differentiation;
cardiomyocyte-like cel s;
α-actin;
troponin T;
stem cel photographs-containing paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(32):5813-5818
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In recent years, embryonic hepatic stem cel s have attracted more attention, but there are few reports on the potential of embryonic hepatic stem cel s to differentiate into cardiomyocyte-like cel s as wel as the related differentiation conditions. OBJECTIVE:To investigate the moderate condition to induce mice embryonic hepatic stem cel s to differentiate into cardiomyocyte-like cel s in vitro with chemical reagents. METHODS:Dimethylsulfoxide in combination with 5-azacytidine with different concentrations and time were used to induce the embryonic hepatic stem cel s of 13.5 days mice and to observe the differentiation effect. RESULTS AND CONCLUSION:Under in vitro conditions, 0.8%dimethylsulfoxide+5μmol/L 5-azacytidine could induce the mouse embryonic hepatic stem cel s to express the specific markers of myocardial cel s, while increasing the concentration of the inducer and extending the induction time could not improve the induction efficacy.
