Effect of the intestinal ischemia/reperfusion injuries on paracrine patterns of BM-MSCs
10.3760/cma.j.issn.1008-6315.2012.13.017
- VernacularTitle:肠道缺血再灌注损伤对骨髓间充质干细胞旁分泌作用的影响
- Author:
Dawei LI
;
Guangzhou GAO
;
Xin LI
;
Tao SUN
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cell;
Ischemia/reperfusion injuries;
Paracrine;
Intestinal epithelial cells
- From:
Clinical Medicine of China
2012;(z1):34-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of the intestinal ischemia/reperfusion injury on paracrine patterns of BM-MSCs in order to provide evidence for BMSCs repair.Methods BMSCs were isolated,cultured,characterized,and stained.The rat intestine ischemia/reperfusion model (IRI) was established.Intestinal epithelial mucosal extracts were obtained either from IRI rat or normal rat.Those two type extracts were added into the culture medium,and BM-MSCs were cultured with the two conditioned medium respectively.The culture medium was collected at 0 h and 24 h after culturing with intestinal epithelial mucosal extracts.The levels of TGF-ot,VEGF,FGF and IGF-1 in the medium were assayed by ELISA.Results The contents of all the growth factors detected in cultured medium with intestinal extracts from IRI rats were significantly higher than in regular rats (P ＜ 0.05).The levels of TGF-α,VEGF in culture medium containing IRI epithelium extracts were significantly higher than that in the medium containing normal intestinal epithelium extracts (P ＜ 0.05)Conclusion BM-MSCs can secret TGF-α,VEGF,FGF,and IGF-1 in the presence of intestinal extracts from IRI rat in the culture.These factors might stimulate the differentiation and proliferation of intestinal epithelial cells through paracrine mechanism.
