Using ITS2 Barcode to Identify Rehmanniae Radix
10.11842/wst.2013.03.018
- VernacularTitle:应用ITS2条形码鉴定中药材地黄＊
- Author:
Dianyun HOU
;
Tianyi XIN
;
Pei YANG
;
Hui YAO
- Publication Type:Journal Article
- Keywords:
Rehmanniae Radix;
ITS2;
DNA barcoding;
identification
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(3):441-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify Rehmanniae Radiuxand its closely related species using the ITS2 barcode and to guarantee the quality and clinical curative effect of this medical material. Method: The sequences has been ana-lyzed and assembled using corresponding software. The Kimura 2-Parameter (K2P) distances were calculated and NJ (neighbor-joining) tree was established based on the K2P methods to identify the Rehmanniae Radix. Results:The length of the ITS2 sequence of Rehmanniae Radix was 231 bp. The average intra-specific genetic distances of Rehmanniae Radix were 0.0004. The average inter-specific genetic distances between Rehmanniae Radix and its closely related species were 0.0312. The minimum inter-specific divergence is larger than the maximum intra-specific divergence. The Rehmanniae Radix can be identified using the NJ trees method. Conclusions: The ITS2 sequence can be used as DNA barcode to identify Rehmanniae Radix and its closely related species, which will lay the foundation for the clinical medication safety of Rehmanniae Radix.
