Cloning and Expression Analysis of HsDXR1 in Huperzia serrata
10.11842/wst.2013.03.002
- VernacularTitle:蛇足石杉1-脱氧-D-木酮糖-5-磷酸还原异构酶（）基因克隆与表达分析＊
- Author:
Hongmei LUO
;
Biao LI
;
Yulin LIN
;
Jingyuan SONG
;
Liu HE
;
Chao SUN
;
Rongtao LI
;
Zhigang HU
- Publication Type:Journal Article
- Keywords:
1-deoxy-D-xylulose-5-phosphate reductoisomerase;
Huperzia serrata;
gene clone;
expression analysis
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(3):342-348
- CountryChina
- Language:Chinese
-
Abstract:
The transcript encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) was discovered from the transcriptome data of Huperzia serrata. The transcript contained an open reading frame with length of 1,440 bp and coded 479 amino acids. The full length of HsDXR1 had been cloned using RT-PCR method. Ac-cording the bioinformatic analysis, the molecular weight of HsDXR1 protein was 51.4961 kDa and the pI was 6.44. No signal peptide and transmembrane site was discovered in HsDXR1, and the protein was most likely to be located in chloroplast. HsDXR1 had the same domain similar to the DXR protein of Arabidopsis and Oryza sativa. The expression level of HsDXR1 was most abundantly in H. serrata stem, followed by root and leaf. This study cloned and analyzed HsDXR1 gene from H. serrata for the first time. The result will provide a foundation for exploring the mechanism of terpene biosynthesis in H. serrata plants.
