Application of COI-based DNA Barcoding for Identifying Animal Medical Materials in the Chinese Pharmacopoeia

VernacularTitle:基于COI条形码序列的《中国药典》动物药材鉴定研究＊
Author: Hui ZHANG ; Hui YAO ; Lina CUI ; He DU ; Zhe LIN ; Xiaochen GAO ; Xue LANG ; Jingyuan SONG ; Kun LUO ; Linchun SHI ; Shilin CHEN
Publication Type:Journal Article
Keywords: Animal medical material; Chinese Pharmacopoeia; COI sequences; DNA barcoding; identification
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(3):371-380
CountryChina
Language:Chinese
Abstract: Medicinal animals are important part of Traditional Chinese medicine resources in China. Cytochrome c oxidase subunit I (COI) was selected as the standard DNA barcoding sequence for animal medical materials. In this study, the 51 animal species from 45 animal medical materials in the Chinese Pharmacopoeia were selected and the intra-specific variation and the inter-specific divergence, the barcoding gap, the identification efficiency of their COI sequences were analyzed. The results showed that the inter-specific divergence is higher than intra-specific distance. The barcoding gap existed between inter-specific sequence divergence and intra-specific dis-tance. The identification efficiencies were 100% both at the genus and species level except the Arthropoda. The cluster dendrogram exhibited that different species distinguished from others. Therefore, COI sequence as a bar-code is suitable to identify the species of animal medical materials in Chinese Pharmacopoeia.