Applying DNA Barcoding Technique to Identify Mori Cortex and Its Adulterants

VernacularTitle:桑白皮及其混伪品的DNA条形码鉴定研究＊
Author: Yongxin XIONG ; Keli CHEN ; Yimei LIU ; Lan WU ; Shiyi LIU
Publication Type:Journal Article
Keywords: DNA barcoding; ITS2; Mori Cortex
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(3):393-396
CountryChina
Language:Chinese
Abstract: To find a new method to identify Mori Cortex and its adulterants by analysis their ITS2 sequence of barcode,total genomic DNA was isolated from Mori Cortex and its adulterants. Nuclear DNA ITS2 sequences were amplified,and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner V3.0. The Kimura 2-Parameter (K2P) distances were calculated us-ing software MEGA 4.0. Identification analyses were performed using BLAST1, Nearest Distance and neighbor-joining (NJ) methods. The results showed the intra-specific genetic distance of Mori Cortex was 0, which were lower than inter-specific genetic distances between Mori Cortex and its closely related species (0.003-0.343). The ITS2 region is an efficient barcode for identification of Mori Cortex and its closely related species, which provides a scientific basis for fast and accurate identification and new method of Mori Cortex.