Identification of Euryales Semen and Its Closely Related Species Using ITS2 Barcode
10.11842/wst.2013.03.012
- VernacularTitle:芡实及其近缘种药材ITS2条形码鉴定研究＊
- Author:
Wei GU
;
Junhua XU
;
Suiyan LI
;
Chengying WU
;
Juanjuan ZHOU
;
Jing SHAO
;
Jianguo CHAO
;
Haishan DENG
;
Qinan WU
- Publication Type:Journal Article
- Keywords:
Euryales Semen;
ITS2;
species identification
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(3):404-409
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify Euryales Semen and its closely related species using the ITS2 barcode. Method:The total genomic DNAs were extracted from twenty samples of Euryales Semen and its closely related species. The ITS2 regions of the samples were amplified and bidirectional sequenced. Obtained sequences were submitted to the GenBank with Sequin 12.3. ITS2 sequences of 102 samples belonging to thirty species were downloaded from GenBank. The 122 ITS2 sequences were aligned and the genetic distances were analyzed with MEGA 5.1. Identification analyses were performed using BLAST1 and nearest distance methods, and were presented intuitively by constructing neighbor-joining (NJ) tree. Result: The length of ITS2 region of Euryales Semen was 214 bp, which was only one haplotype. There was significant divergence of the ITS2 regions among the samples. The NJ tree showed that Euryales Semen could be obviously differed from its closely related species, which had good 408 monophyly. Conclusion: ITS2 regions as a DNA barcode can stably and accurately distinguish Euryales Semen from its closely related species and also provide a new technique to ensure clinical safety in utilization of tradi-tional Chinese medicines. The new exploration could broaden the application of DNA barcoding technology in identification of Traditional Chinese Medicine.
