The Identification of Uygur Medicine Dracocephalum Moldavica L. and Its Sibling by ITS2 Sequence
10.11842/wst.2013.03.014
- VernacularTitle:维吾尔药材香青兰DNA条形码鉴定方法的研究＊
- Author:
Congzhao FAN
;
Xiaojin LI
;
Jun ZHU
;
Guopin WANG
;
Hailong SONG
- Publication Type:Journal Article
- Keywords:
Dracocephalum moldavica L.;
ITS2;
sibling species;
identification
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(3):415-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the DNA barcode identification method of Uygur Medicine Dracocephalum mol-davica L. Methods: D. moldavica L. and its ten sibling species of twenty five samples was amplified by ITS2 se-quence, after sequencing and comparing the intraspecific and interspecific variation, we using K2P and NJ meth-ods to analysis the their relationship, then compare the secondary structure. Results: D. Moldavica (KF041160, KF041163, KF041168, KF041169) from Xinjiang without variation in intraspecies, but there are two 2 variation sites in D. moldavica (AY506659) from GenBank. By NJ method, D. moldavica can be distinguished with their sibling species. Also, D. nutans L., D. bipinnatum Rupr. and D. integrifolium Bge. can be distinguished with oth-er sibling species. Conclusion: ITS2 barcode sequence was able to identify D. moldavica and its sibling species, which provides an effective way for the molecular identification of Uygur Medicine D. moldavica.
