The application and significance of gene scanning in the clonality analysis of Ig/TCR gene rearrangements in new cases of acute lymphocytic leukemia
10.3760/cma.j.issn.1009-9158.2013.03.008
- VernacularTitle:基因扫描在初诊急性淋巴细胞白血病患儿Ig/TCR基因重排克隆性分析中的应用
- Author:
Huiling LIN
;
Yanmei LI
;
Tiezhen YE
;
Yan LI
;
Huanhuan ZHU
;
Liyi JIANG
- Publication Type:Journal Article
- Keywords:
Precursor cell lymphoblastic leukemia-lymphoma;
Gene rearrangement,T-lymphocyte;
Clone cells;
Neoplasm,residual
- From:
Chinese Journal of Laboratory Medicine
2013;(3):228-232
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of gene Scanning in the clonality analysis of Ig/TCR gene remrangement in children with newly diagnosed acute lymphoblastic leukemia (ALL),and the relationship between the clinical characteristics of ALL and the type of Ig/TCR gene rearrangement.Methods The research was the clinical experimental study.Selected 86 cases of children with ALL who were diagnosed and treated in Department of Hematology-oncology of Guangzhou Women and Children's Medical Center,and All cases were confirmed by bone marrow cell morphology and flow cytometric immunophenotyping.Used multiplex PCR to detecte Ig/TCR gene rearrangements in children with newly diagnosed ALL.Applied gene scanning to analyze the clonality of Ig/TCR gene rearrangement.Results There were 83 cases detected 1 or more than 1 types of Ig/TCR gene rearrangements in 86 cases (96.5％),with 2.52 types each case.There were 56 cases detected at least one monoclonal Ig/TCR gene rearrangement in 61 cases analyzed by gene scanning (91.8％).The detectable rate for lgH,Igκ,TCRγ and TCRδ were 27.91％,27.91％,19.77％ and 24.42％ respectively in 172 of Ig/TCR gene rearrangement.Monoclonal,oligoclonal and polyclonal composition was 58.1％,30.8％ and 11.1％ respectively,the monoclonal as the main component.There was no significant difference between the types and clonality of Ig/TCR gene rearrangement and the clinical characteristics of children with newly diagnosed ALL (P ＞ 0.05).Conclusions Gene scanning can analyze clonality of the Ig/TCR gene rearrangement conveniently and rapidly,thus,it can be possible to select the stable targets for quantitative detection of minimal residual disease minimal residual disease.There is no significant difference between the types and clonality of Ig/TCR gene rearrangement and clinical characteristics of children with newly diagnosed.
