The effects of electromagnetic fields on osteogenesis during co-culture of bone marrow mesenchymal stem cells with osteoblasts
10.3760/cma.j.issn.0254-1424.2013.04.003
- VernacularTitle:共培养条件下电磁场干预对大鼠成骨细胞及骨髓间充质干细胞成骨分化的影响
- Author:
Jizhe YU
;
Yong YANG
;
Zhaoxu LIU
;
Mingyu SONG
;
Yang LIU
;
Hua WU
- Publication Type:Journal Article
- Keywords:
Electromagnetic fields;
Osteoblasts;
Bone marrow;
Mesenchymal stem cells;
Coculturing
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2013;(4):250-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of electromagnetic fields (EMFs) on osteogenesis during co-culture of bone mesenchymal stem cells (BMSCs) with osteoblasts in rats.Methods BMSCs and osteoblasts were isolated from Sprague-Dawley rats and cultured.Sub-cultured osteoblasts and BMSCs were seeded in transwell cell-culture-chamber polyester inserts to establish the co-culture system.The co-cultures were then randomly divided into a normal co-culture group and a group exposed to an EMF.Single-cultured BMSCs and osteoblasts were set as a single culture group.The EMF group was exposed to an EMF for 4 hours per day.On the 14th day,cell culture plates or inserts were randomly selected for total RNA extraction and measurement of the mRNA expression levels of Runt-related transcription factor 2,transcription factor 7,alkaline phosphatase,collagen type Ⅰ,bone morphogenetic protein 2 (BMP-2) and bone gamma-carboxyglutamate protein (Osteocalcin gene,OC gene) using real-time PCR assays.Cell culture dishes or inserts were also randomly chosen for Alizarin red staining to detect mineralized nodules.Results The level of osteogenic gene expression in single-cultured BMSCs and osteoblasts was low,while it was much higher in the co-culture group.The level of gene expression in the EMF-exposed and co-cultured group was even higher.Alizarin red staining also showed that calcium mineralized modules had increased in the stimulated,co-cultured system compared with the unstimulated,co-cultured cells.Conclusion EMF exposure can promote osteogenic differentiation of BMSCs and osteoblasts when they are co-cultured.BMP2-mediated cellular interaction might play an important role in osteogenic differentiation induced by EMF exposure.
