Protective effect of MG132 on ischemia-reperfusion injury after pancreaticoduodenal transplantation in rats
10.3760/cma.j.issn.0254-1785.2013.06.009
- VernacularTitle:蛋白酶体抑制剂MG132减轻大鼠移植胰腺缺血再灌注损伤的作用
- Author:
Li ZHANG
;
Xueyun ZHAO
;
Shengchun LIU
- Publication Type:Journal Article
- Keywords:
Rats;
Pancreas transplantation;
Reperfusion injury;
Proteasome inhibitor;
NF-κB
- From:
Chinese Journal of Organ Transplantation
2013;(6):358-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of Proteasome inhibitor MG132 on ischemia-reperfusion injury (IRI) after pancreas transplantation in rats,and the possible mechanism.Method Fifteen normal SD rats were allocated into the sham operation group.In the allogenic male SD rats,the model of pancreas transplantation was established.The recipients were divided into another two groups (n =15 each) at random:IRI group and the MG132 pretreatment group.Serum amylase level was determined at 1,3 and 6 h after the operation.Pancreas samples were harvested at the same time for pathological study by light microscopy.The expression of NF-κB P65 protein in the pancreas was detected by using Western blotting.The expression of TNF-α and ICAM-1 in the pancreas was detected by using immunohistochemistry and RT-PCR.Result Tissue damage on IRI group was more severe than in sham operation group.The level of serum amylase,and the expression of P65,TNF-α and ICAM-1 were higher in IRI group than those in sham operation group (P＜0.05).Tissue damage in MG132 pretreatment group was milder than in IRI group.The level of serum amylase,and the expression of P65,TNF-α and ICAM-1 were lower in MG132 pretreatment group than in IRI group (P＜0.05).Conclusion MG132 pretreatment can alleviate the pancreas IRI after pancreas transplantation,probably by inhibiting the activation of NF-κB,and the inhibition effect can down-regulate the expression of TNF-α and ICAM-1.
