Ventilation with cooled carbon monoxide protects non-heart-beating donor rat lungs against worm ischemic injury
10.3760/cma.j.issn.0254-1785.2013.05.012
- VernacularTitle:低温下一氧化碳通气对无心跳大鼠供肺的保护机制研究
- Author:
Qiankun CHEN
;
Gening JIANG
;
Chang CHEN
;
Xiaofeng CHEN
;
Jiaan DING
- Publication Type:Journal Article
- Keywords:
Carbon monoxide;
Hypothermia;
Rats;
Lung transplantation;
Ischemia
- From:
Chinese Journal of Organ Transplantation
2013;(5):299-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective Lungs from non-heart-beating donors for transplantation require protection against warm ischemic damage.This study investigated the preservative effect of Ventilation with cooled carbon monoxide during warm ischemia in non-heart-beating donor rat lungs.Method 18 rats were divided into a CO group (n =6),which received ventilation with low-dose carbon monoxide at normal temperature during a 4-hour warm ischemic period; a Control group (n =6),which received no ventilation at normal temperature; a cooling CO group (n =6),which received ventilation with cooled carbon monoxide.PaO2,Myeloperoxidase (MPO) activity,Bronchoalveolar lavage (BAL) neutrophil count and the wet-to-dry (W/D) lung weight ratio were recorded in every group.Quantitative real-time RT-PCR was used to analysis the expression of IL-1β and caspase 3 mRNA in graft lung tissures.Result Endobronchial temperatures and lung surface temperatures in the Cooling CO group were lower than those in the corresponding Control group and CO group (P＜ 0.01).Lower wet/dry lung weight ratio,MPO activity,BAL neutrophil count,expression of IL-1β and caspase 3 mRNA in graft lung tissures were seen in the Cooling group compared with the Control group and CO group (P＜0.05).Conclusion Ventilation with cooled carbon monoxise can decrease lung temperature and improve the protecting effect on non-heart-beating donor rat lungs againt worm ischemic injury by inhibiting the expression of proimflammatory factor IL-1β and apoptosis-associated gene caspase 3.
