The endothelial function under various blood glucose levels and the protective effect of PGC-1α on macrovasculature in patients with diabetes mellitus
10.3760/cma.j.issn.1000-6699.2013.06.008
- VernacularTitle:不同血糖情况下的血管内皮功能与PGC-1α对糖尿病大血管的保护作用
- Author:
Wensheng LU
;
Buduo SHI
;
Qun HUANG
;
Yuanmei LIN
;
Chuan YANG
;
Qin HUANG
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus,type 2;
Vascular endothelial function;
Flow-mediated vasodilatation;
Glyceryl trinitrate dilatation;
Carotid intima-media thickness;
PPARγ coactivator 1α
- From:
Chinese Journal of Endocrinology and Metabolism
2013;(6):479-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the vascular endothelial function in individuals with normal glucose tolerance (NGT),impaired glucose tolerance (IGT),and type 2 diabetes mellitus,and to study the role played by PPARγcoactivator 1 α (PGC-1 α) in diabetic macrovascular function.Methods A total of 94 subjects with NGT,87with IGT,and 306 with type 2 diabetes mellitus were recruited in the study.Carotid flow-mediated vasodilatation (FMD),glyceryl trinitrate dilatation (GTN),and carotid intima-media thickness (CIMT) were assessed by highresolution B-mode color vascular Doppler instrument.Finally,correlation analysis was conducted.NO,Akt-Ser473phosphorylation levels,IL-β,and PGC-1 α mRNA expression levels were measured by means of nitrate reductase,western blot,and reverse transcriptase real-time fluorescence quantitative PCR assay methods in peripheral blood mononuclear cells respectively.Results FMD in newly-diagnosed type 2 diabetic group was significantly lower than that in NGT and IGT groups(P＜0.01).GTN and CIMT showed no difference.PGC-1 α mRNA transcription level in diabetic group was significantly lower than that in non-diabetic groups (P＜0.01).Correlation analysis showed that PGC-1 α was positively correlated with FMD and GTN (P＜0.05 or P＜0.01).Logistic regression analysis revealed that PGC-1 α was independently associated with FMD (P ＜ 0.01) after adjusting other influencing factors.Conclusion FMD is a more sensitive early warning indicator of endothelial function than CIMT and GTN.As indices of atherosclerosis,CIMT and GTN are more or less focused on the progression of macrovascular complications.PGC-1 α is a protective factor of macro-vasculature in diabetes mellitus,and its effect may be achieved through the protection of the vascular endothelium.
