Effect of metformin on expression of SIRT3 in skeletal muscle of rats with type 2 diabetes

VernacularTitle:二甲双胍对2型糖尿病大鼠骨骼肌SIRT3表达的影响
Author: Yuping SONG ; Chong HAN ; Jingli SHI ; Ying WU ; Zhimin LIU
Publication Type:Journal Article
Keywords: Metformin; SIRT3; Diabetes mellitus,type 2; Rats
From: Chinese Journal of Endocrinology and Metabolism 2013;(5):427-429
CountryChina
Language:Chinese
Abstract: [Summary] Type 2 diabetic rat model accompanied by insulin resistance was established by high fat/high glucose diet and streptozotocin.Following metformin treatment for 4 weeks,realtime PCR and Western blot were used to detect the expressions of SIRT3 mRNA and protein in skeletal muscle tissue,respectively.The results showed that insulin sensitivity,superoxide dismutase (SOD),and glutathione (GSH) levels were significantly reduced in diabetic group compared with the control group (P＜0.05),while methane dicarboxylic aldehyde (MDA) level was increased (P＜0.05),along with the decreased expressions of SIRT3 mRNA and protein in skeletal muscles (P＜0.01).After metformin treatment,the insulin sensitivity,SOD,GSH,and SIRT3 mRNA and protein levels were significantly increased (P＜ 0.05 or P＜ 0.01),while MDA level was decreased (P ＜ 0.05),suggesting that metformin may ameliorate insulin resistance via upregulating SIRT3 expression in skeletal muscles of diabetic rats.