Change of intracellular calcium concentration in prostate smooth muscle cells of SD rats with chronic abacterial prostatitis under high potassium solution
10.3760/cma.j.issn.1000-6702.2013.03.017
- VernacularTitle:高钾溶液对慢性非细菌性前列腺炎SD大鼠前列腺平滑肌细胞内钙离子浓度的影响及意义
- Author:
Jianhui LIU
;
Chaozhao LIANG
;
Xiansheng ZHANG
;
Zongyao HAO
;
Song FAN
;
Zhen ZHANG
;
Kexiao WANG
- Publication Type:Journal Article
- Keywords:
Prostatitis;
Myocytes,smooth muscle;
Calciumion
- From:
Chinese Journal of Urology
2013;(3):224-228
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of intracellular calcium ion concentration in prostate smooth muscle cells of SD rats with chronic abacterial prostatitis under high potassium solution.Methods SD rats were divided into experiment group and control group.The CP model was set up by castration and estradiol injection.The PSMC was cultured and purified in vitro.Laser confocal scanning microscope was used after the ceils were incubated with Quest Fluo-8TM.The cells were treated with high potassium solution,and the change of fluorescence intensity was observed.Results The pathologic specimens of the experiment group showed typical pathologic characteristics of chronic prostatitis under light microscope,the control group without inflammation performance.Using immunocytochemistry method confirmed that the experiment group and the control group were prostate smooth muscle cells.The change of fluorescence intensity of [Ca2+] i in the experiment group and control group in the high potassium solution was 27.86 ± 9.88 and 7.61 ± 4.31.There were statistically significant differences between the two groups (P ＜ 0.01).Conclusions High potassium solution cause intracellular calcium ion concentration increased.
