Formulation of paclitaxel loaded PLGA nanoparticle and it's cytotoxicity on bladder cancer

VernacularTitle:转运紫杉醇的PLGA纳米粒对膀胱癌的细胞毒作用
Author: Shihua JIN ; Ningchen LI ; Zhihong ZHANG ; Yanqun NA
Publication Type:Journal Article
Keywords: Nanoparticle; Paclitaxel; Bladder neoplasms; Carcinoma
From: Chinese Journal of Urology 2013;(3):219-223
CountryChina
Language:Chinese
Abstract: Objective To formulate paclitaxel loaded polymer nanoparticle and evaluate it's application in treatment of bladder.Methods Paclitaxel loaded Poly (lactide-co-glycolide) (PLGA) nanoparticles were formulated with microemulsion method,Polyvinyl alcohol(PVA) was used as surfactant.Transferrin (Tf) was used to modify the nanoparticles.The size,Z-potential,drug loading,drug release,cytotoxicity of bland nanoparticles and paclitaxel-loaded nanoparticles on bladder cancer cell line J-82 were measured.Results The size of nanoparticles was about 200 nm,Z-potential was-24 mV,drug loading was about 6.5％ (w/w),cumulative drug release showed two phase curve.The size of Tf modified nanoparticles was a little bigger than no modified nanoparticles.The Z-potential,drug loading,drug release was similar.Two kinds of blank nanoparticles showed no cytotoxicity on bladder cancer cell line J-82.However,both paclitaxel-loaded nanoparticles had significantly higher cytotoxicity on J-82 compared to paclitaxel solution.Conclusions PLGA nanoparticle is a promising drug delivery vehicle,which could significantly improve the anticancer effect of paclitaxel on bladder cancer.