Radioprotection effect of lipoic acid amino acid salts on L20 and AHH-1 cells
10.3760/cma.j.issn.0254-5098.2013.03.013
- VernacularTitle:硫辛酸氨基酸盐对L02和AHH-1细胞辐射防护作用的研究
- Author:
Li FAN
;
Deqing CHEN
;
Qingjie LIU
;
Jiangbin FENG
;
Xue LU
;
Hua ZHAO
;
Jianxiang LIU
- Publication Type:Journal Article
- Keywords:
Lipoic acid amino acid salt;
Lipoic acid arginine salt;
ESR;
CCK-8;
Radioprotection
- From:
Chinese Journal of Radiological Medicine and Protection
2013;(3):269-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective For developing safe and effective anti-radiation new drugs,the effects of different lipoic acid amino acid salts on radiosensitivity were investigated.Methods The free radical scavenging ability of the above salts was evaluated in Fenton system.CCK-8 assay and flow cytometry assay were performed to evaluate the survival rate of L02 and apoptosis of AHH-1 after γ-ray irradiation,respectively.Results The lipoic acid arginine salt had the best ability of scavenging free radicals in Fenton system with an IC50 of 8.40 μ moL/ml.The survival assay showed that lipoic acid amino acid salts had better stability and equal ability in radioprotection (P ＞ 0.05) compared with lipoic acid.The apoptosis assay indicated that all lipoic acid amino acid salts could inhibit radiation-induced apoptosis,where lipoic acid arginine salt was more effective (t =-6.67,P ＜ 0.01).Conclusions Lipoic acid arginine salt has good radioprotection effect on L02 and AHH-1 cells by scavenging free radicals.
