Radiosensitization effect of gold nanoparticles modified by sodium glycididazole on lung adenocarcinoma cell A549
10.3760/cma.j.issn.0254-5098.2013.03.012
- VernacularTitle:甘氨双唑钠修饰纳米金对肺腺癌细胞A549的放射增敏研究
- Author:
Xinye NI
;
Nong QIAN
;
Tao LIN
;
Bin NIE
;
Zhiqiang SUN
;
Suping SUN
- Publication Type:Journal Article
- Keywords:
Sodium glycididazole;
Gold nanoparticles;
Radiosensitization;
Lung adenocarcinoma cell/AS49
- From:
Chinese Journal of Radiological Medicine and Protection
2013;(3):265-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radioseusitization effect of gold nanoparticles modified by sodium glycididazole.Methods The sodium glycididazole was connected to gold nanoparticle,in dimension of about 18 nm,that had been modified with polyethylene glycol.The nanoparticle-swallowing efficiency of lung adenocarcinoma A549 cells was observed by a scanning electron microscope.Cells were divided into four groups:sodium glycididazole group,gold nanoparticles group,sodium glycididazole-gold nanoparticles group,and no drug control group.The radiosensitivity was detected by MTT and colony formation assays.Results Sodium glycididazole-gold nanoparticles could enter the cell cytoplasm and nucleus.The concentration of 0.003 mg/ml gold nanoparticles and sodium glycididazole-gold nanoparticles had no obvious cytotoxic effect.After irradiation of 2,4,6,8 Gy,the cell survival of the sodium glycididazole-gold nanoparticle group was significantly lower than that of the other three groups (F =4.8,14.5,5.7,7.6,P ＜0.05) and the D0 and Dq values of the sodium glycididazole-gold nanoparticle group were significantly lower than those of other three groups.Conclusion Sodium glycididazole-gold nanoparticles can enhance the radiosensitivity of lung adenocarcinoma cells.
