Roel of Lys63-linked TAK1 poly-ubiquitination at lysine 158 in radiation-induced NF-κB activity
10.3760/cma.j.issn.0254-5098.2013.03.004
- VernacularTitle:TAK1赖氨酸158位点发生Lys63连接多聚泛素化在辐射诱导NF-κB激活过程中的作用
- Author:
Xiang ZHU
;
Baoshan CAO
;
Zhaohui ZHANG
;
Liwen MA
;
Xu SU
;
Li LIANG
- Publication Type:Journal Article
- Keywords:
Radiation effect;
Transforming growth factor-β-activating kinase 1;
Polyubiquitination;
Nuclear factor-kappa B;
Signaling pathway
- From:
Chinese Journal of Radiological Medicine and Protection
2013;(3):235-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of the transforming growth factor-β-activating kinase 1 (TAK1) poly-ubiquitination in radiation-induced NF-κB activation.Methods FLAG-TAK1 and HA-UbK63 plasmids were transiently transfected into HEK-293T cells.The radiation effect on TAK1 ubiquitination was detected by immunoprecipitation and Western blot,which was further confirmed in FLAG-TAK1 HeLa stable cell line.Results At 1 h after irradiation,Lys 63-1ink TAK1 ubiquitination was induced in the HEK-293T cells with plasmids transfection.This result was further confirmed by using a FLAG-TAK1 stably expressed HeLa cell line.Radiation-induced IKKs and p38 phosphorylation were greatly impaired in MEFs reconstituted with TAK1 K158R mutant compared to ones with wild-type TAK1.Conclusions Lys63-1inked TAK1 poly-ubiquitination at Lys-58 plays a key role in the process of radiation-induced NF-κB activation.
