Detection of BRAF in the synovial fluid and its clinical significance in rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2013.07.005
- VernacularTitle:鼠科肉瘤病毒癌基因同源物B1蛋白在类风湿关节炎滑液中的表达及其临床意义
- Author:
Jinxia ZHAO
;
Lin SUN
;
Yingjian ZHANG
;
Xia ZHANG
;
Ru LI
;
Xiangyuan LIU
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Synovial fluid;
v raf murine sarcoma viral oncogene homologue B1
- From:
Chinese Journal of Rheumatology
2013;(7):446-449
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect v raf murine sarcoma viral oncogene homologue B1 (BRAF) in the synovial fluid of rheumatoid arthritis (RA) and to investigate its clinical significance in RA.Methods Synovial fluid samples were obtained from patients with RA and osteoarthritis (OA).Serum samples were obtained from patients with RA,OA and heathy controls.The presence of BRAF in the synovial fluid and sera were examined by enzyme-linked immunosorbent assay (ELISA).Western blotting was used to detect the expression of BRAF protein in the synovial tissue of RA and OA.The associations between the BRAF and the clinical features and laboratory parameters of RA were evaluated.Data analysis were performed using t test and Spearman's association analysis.Results ① The level of BRAF in the synovial fluid of RA [(84±59) ng/ml] was significantly higher than OA [(38±41) ng/ml] (t=3.290,P=0.002).② The level of BRAF in the sera of RA patients [(22.0±12.5) ng/ml] was also higher than OA [(6.8±7.5) ng/ml,t=3.882,P＜0.01] and healthy controls [(4.8±2.2) ng/ml,t=6.766,P＜0.01].③ In RA patients,the BRAF protein level in the synovial fluid [(102±52) ng/ml] was significantly higher than that in the serum [(21±12) ng/ml] (t=-4.316,P=0.003).④The expression level of BRAF in the synovial tissue of RA (0.284±0.045) was higher than that in OA patients (0.191±0.013,t=3.169,P=0.034).⑤ The level of BRAF in the synovial fluid had a negative correlation with disease duration (r=-0.40,P=0.019) and a positive correlation with rheumatoid factor (RF) levels (r=0.37,P=0.03).Conclusion The presence of BRAF in the synovial fluid and synovium of RA indicates that BRAF may play a role in the pathogenesis of RA,especially in the early stage.
