Ultrasound-targeted microbubble destruction combined with polyethylenimine mediated Bax gene silence in H9c2 cells
10.3760/cma.j.issn.1004-4477.2013.05.023
- VernacularTitle:超声靶向微泡破裂联合聚乙烯亚胺介导大鼠心肌母细胞Bax基因沉默
- Author:
Jiamei JIN
;
Yuhui ZHANG
;
Ye ZHU
;
Liangping ZHANG
;
Ming ZONG
;
Ming CHEN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
H9c2 cells;
RNA interference;
Gene silencing
- From:
Chinese Journal of Ultrasonography
2013;(5):438-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To silence Bax gene in H9c2 cells by ultrasound-targeted microbubble destruction(UTMD) combined with polyethylenimine.Methods The shRNA expression plasmid targeting Bax gene was co-treated with microbubbles and polyethylenimine.Then the mixture added to H9c2 cells were irradiated by ultrasound.Grouping:① plasmid group;② Lipofectamine2000 + plasmid group; ③ UTMD + plasmid group;④ PEI + UTMD + plasmid group;⑤ PEI + SonoVue + plasmid group (without ultrasound irradiation).The transfection efficiency was assessed by fluorescence microscope and FCM.The expression of Bax mRNA and protein were detected by RT-PCR and Western Blot.Results Bax shRNA wasn't destroyed by ultrasound exposure.The transfection efficiency of PEI + UTMD + plasmid group was higher than any other groups obviously (P ＜0.05).The result of RT-PCR and Western Blot showed inhibition efficiency of Bax mRNA and protein expression in PEI + UTMD + plasmid group were (69.41 ± 4.91) ％ and (64.09 ± 2.38)％ separately,which were higher than any other groups significantly (P ＜0.05).Conclusions UTMD combined with polyethylenimine can inhibit the expression of Bax gene in H9c2 cells effectively.
