Studies on the changes and clinical significance of peripheral blood T lymphocyte Subsets in 386 Patients with Hand, Foot and Mouth Disease
10.3760/cma.j.issn.1008-6315.2013.06.034
- VernacularTitle:手足口病患儿外周血T淋巴细胞亚群在病程中的变化及临床意义
- Author:
Linchun SUN
;
Lanfang ZHANG
;
Li ZHANG
;
Zheng HU
- Publication Type:Journal Article
- Keywords:
Hand,foot and mouth disease;
Children;
T lymphocyte subsets;
Cellular immunity;
Flow cytometry
- From:
Clinical Medicine of China
2013;(6):660-663
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the changes of peripheral blood T lymphocyte subsets in patients with hand,foot and mouth disease (HFMD) and investigate its clinical significance.Methods Three hundred and eighty-six patients with HFMD and 78 healthy children were detected the peripheral blood T lymphocyte subsets levels using the flow cytometry (FCM).The patients were divided into 325 common patients and 61 critically ill patients according to the severity of disease.Among the 386 patients,264 were in infection stage and 122 were in recovery stage.Compared the differences of peripheral blood T lymphocyte subsets levels in patients and healthy controls,common patients and critically ill patients,infection patients and recovery patients respectively.Results The patients' numbers of CD3 +,CD4 +,CD8 + cells and CD4 +/CD8 + ratio were (47.4 ± 10.6) ％,(24.0 ±8.0) ％,(23.2 ± 7.4) ％ and 1.0 ± 0.5 respectively.These parameters were significantly different with those in the control group ((68.4±7.5)％,(38.2 ±6.9)％,(18.8 ±4.6)％ and 2.0 ±0.4 respectively;t =3.16,4.62,2.23 and 2.51 respectively,P ＜ 0.05).The common patients' numbers of CD3 +,CD4 +,CD8 + cells and CD4+/CD8 + ratio were (53.0 ±9.8)％,(26.5 ±7.9)％,(20.4±7.5)％and 1.3 ±0.6,which had significant differences compared with those of the critically ill patients ((42.3 ± 10.9) ％,(18.7 ± 4.7) ％,(24.2 ± 7.4) ％ and (0.7 ± 0.4) respectively; t =2.25,2.47,1.91 and 2.32 respectively,P ＜ 0.05).The infection patients' CD3 +,CD4 +,cell numbers and CD4 +/CD8 + ratio were (45.3 ± 10.5) ％,(21.6 ± 8.4) ％and 0.9 ± 0.5.These parameters were significantly different with those of the recovery patients ((54.7 ±9.9)％,(27.5 ± 9.1)％ and 1.4 ± 0.6 respectively; t =2.41,1.87 and 2.92 respectively,P ＜ 0.05).Conclusion The cellular immunity of patients with HFMD was impaired.The T lymphocyte function was suppressed,and the level of suppression was positively correlated to severity of the illness.
