Prokryotic expression of CFP-10 protein of Mycobacterium tuberculosis

VernacularTitle:结核分枝杆菌早期分泌蛋白CFP-10的原核表达
Author: Shisheng FENG ; Jianqin LIANG ; Jinhe WANG ; Xueqiong WU
Publication Type:Journal Article
Keywords: Mycobacterium tuberculosis/genetics; Bacterial proteins/genetics; Genetic vectors
From: Journal of Chinese Physician 2013;(3):324-327
CountryChina
Language:Chinese
Abstract: Objective To construct recombinant plasmid containing CFP-10 gene of Mycobacterium tuberculosis(MTB).Methods The gene fragment of CFP-10 was amplified by PCR from Mycobacterium tuberculosis H37Rv genomic DNA and cloned to pET-32a(+) vector.The recombinant plasmid pET-32a-CFP-10 was transformed into E.coli BL21 (DE3) and induced by IPTG.Results CFP-10 gene fragment was amplified from genomic DNA of Mycobacterium tuberculosis H37Rv strain,and thepET-32a(+) prokaryotic recombinant plasmid was constructed successfully.The recombinant protein was expressed with the induction of IPTG.Conclusions The prokaryotic expression vector for CFP-10 was successfully constructed and the recombinant protein was highly expressed in E.coli BL21 (DE3),which lays a foundation for its subsequent immunological function study.