Andrographolide inhibits cervical cancer cell metastasis via NF-κB pathways
10.3760/cma.j.issn.1008-1372.2013.03.005
- VernacularTitle:穿心莲内酯通过NF-κB途径抑制人宫颈癌细胞转移
- Author:
Li PENG
;
Fenghui ZHONG
;
Yuan DENG
;
Lu HE
- Publication Type:Journal Article
- Keywords:
ANDROGRAPHOLIDE/pharmacology;
NF-kappa B;
Uterine cervical neoplasms/pathology;
Tumor cells,cultured/pathology
- From:
Journal of Chinese Physician
2013;(3):304-306
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the inhibitory effect of andrographolide (AD) on the migration and invasion of human cervical cancer cells,and investigate the molecular mechanisms underlying the inhibitory effect of AD.Methods Human cervical cancer cell lines HeLa cells were cultured in vitro.After induced by 100 nmol/L of phorbol-12-myristate-13-acetate(PMA) for 24 h,different concentration of AD was co-incubated for another 24 h.Cell viability was detected by methyl thiazolyl tetrazoliym (MTT) assay.Chamber invasion assay was used to observe the migration and invasion,respectively.Expression of matrix metalloproteinase-9 (MMP-9) and translocation of nuclear factor kappa B (NF-κB) p65 subunit were detected by Western blot.Results The concentration (0 ～ 10 μmol/L) of AD exhibited a non-cytotoxic effect on HeLa cells.10 μmol/L of AD decreased PMA-induced migration by 49％ and invasion by 52％.Western blot showed that AD suppressed PMA-induced MMP-9 protein expression in a dose-dependent manner.Treatment of AD could decrease the translation of p65 in HeLa cells.Conclusions AD inhibits MMP-9 expression mediated by NF-κB,which lead to the suppression of migration and invasion of cervical cancer cells.
