Naltrexone influences protein kinase C epsilon and integrin alpha7 activity in SH-SY5Y neuroblastoma cells.
- Author:
Dong Yul OH
1
;
Kyoung Hwa JUNG
;
Byung Hwan YANG
;
Jun Suk LEE
;
Ihn Geun CHOI
;
Young Gyu CHAI
Author Information
1. Department of Psychiatry, Kwandong University, Gangneung 210-701, Korea.
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
alcohols;
DNA microarray;
ITGA7 protein;
human;
naltrexone;
neuroblastoma;
protein kinase C
- MeSH:
Antigens, CD/*metabolism;
Cell Line, Tumor;
Comparative Study;
DNA, Complementary/genetics;
Humans;
Integrin alpha Chains/*metabolism;
Naltrexone/*pharmacology;
*Neuroblastoma/enzymology/metabolism/pathology;
Oligonucleotide Array Sequence Analysis;
Protein Kinase C-epsilon/*metabolism;
Research Support, Non-U.S. Gov't;
Reverse Transcriptase Polymerase Chain Reaction;
Time Factors
- From:Experimental & Molecular Medicine
2006;38(1):100-106
- CountryRepublic of Korea
- Language:English
-
Abstract:
Alcohol influences the neuroadaptation of brain cells where receptors and enzymes like protein kinase C (PKC) exist. Naltrexone acts on opioid receptors. However, other mechanisms of action remain unknown. We prepared SH-SY5Y neuroblastoma cells, and fed them with 150 mM ethanol for 72 h followed by treatment with naltrexone for 24 h. We performed microarray analysis and reverse transcriptase-polymerase chain reaction. Our results showed that PKC epsilon increased 1.90 times and showed an overall decreasing pattern as time increased. Phosphorylated ERK also increased 2.0 times according to the change of PKC epsilon. Integrin alpha7 increased 2.32 times and showed an increasing pattern as time increased. In conclusion, naltrexone influences PKC epsilon neuronal signaling system and endothelial adhesion molecule integrin alpha7 in addition to the well-known opioid system.
