Effect of orphan nuclear receptor NR4A1 expression on oxygen-glucose deprivation-induced apoptosis in cultured rots cerebellar granule neurons
10.3760/cma.j.issn.1673-4165.2013.03.004
- VernacularTitle:孤儿核受体NR4A1表达对氧-葡萄糖剥夺诱导培养大鼠小脑颗粒神经元凋亡的影响
- Author:
Guodong XIAO
;
Songming CHENG
;
Tao SUN
;
Yongjun CAO
- Publication Type:Journal Article
- Keywords:
Nuclear Receptor Subfamily 4,Group A,Member 1;
Neurons;
Cerebellum;
Cells,Cultured;
Brain Ischemia;
Oxygen;
Glucose;
Apoptosis;
Caspase 3;
Cytochromes c;
Neuroprotective Agents;
Rats
- From:
International Journal of Cerebrovascular Diseases
2013;(3):181-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of orphan nuclear receptor NR4A1 expression on oxygenglucose deprivation (OGD)-induced apoptosis in cultured rat cerebellar granule neurons and its possible mechanisms.Methods Primary rat cerebellar granule neurons were cultured for 7 to 8 days,and then treated with OGD.The activity of cultured rat cerebellar granule neurons was assessed by methyl thiazolyl tetrazolium (MTT) assay,apoptosis was detected with flow cytometry,the expressions of NR4A1,caspase-3 and cytochrome c were determined by Western blot analysis,NR4A1 mRNA expression was detected with real-time polymerase chain reaction.The rat cerebellar granule neurons were transfected with lentiviral vector-encoding rat NR4A1.The apoptotic rates and expressions of caspase-3 and cytochrome c in rat cerebellar granule neurons transfected with NR4A1 were detected after OGD.Results The activity of rat cerebellar granule neurons decreased significantly,the apoptotic rate increased significantly,the expressions of NR4A1 mRNA and protein as well as caspase-3 and cytochrome c incrased significantly along with the OGD time.NR4A1 was overexpressed,apoptosis rate was significantly reduced in rat cerebellar granule neurons transfected with NR4A1.The expressions of caspase-3 and cytochrome c were significantly reduced in the rat cerebellar granule neurons transfected with NR4A1 after OGD.Conclusions NR4A1 overexpression may reduce OGD-induced apoptosis in rat cerebellar granule neurons by downregulating the expressions of caspase-3 and cytochrome c.
