Expression of voltagE-gated sodium channel SCN5A/Nav1.5 in human ovarian cancer and its significance
10.3781/j.issn.1000-7431.2009.07.011
- VernacularTitle:电压门控钠通道SCN5A/Nav1.5在卵巢癌中的表达及意义
- Author:
Rui GAO
;
Yi SHEN
;
Shuxiang XU
;
Ming LEI
;
Zehua WANG
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Sodium channels,voltagE-gated;
Neoplasm invasiveness;
Neoplasms metastasis;
Tetrodotoxin
- From:
Tumor
2009;(7):654-658
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The different subtypes of voltagE-gated sodium channels (VGSCs) are known to correlate with the migration of many malignant cancers. This study was to investigate the significance of functional expression of SCN5A/Nav1.5 in human ovarian cancer and its effects on migration capability of ovarian cancer cells in vitro. Methods: Sodium indicator SBFI and immunofluorescence method were used to detect the distribution of intracellular Na+. Real-time PCR, Western blotting, and immunohistochemistry were used to detect the mRNA and protein expression of SCN5A/Nav1.5. The effect of specific voltagE-gated sodium channels inhibitor tetrodotoxin (TTX) on cell viability was measured by CCK-8 kit. The migration and invasion of ovarian cancer cell lines SKOV-3 were tested by Transwell chamber assay. Results:SCN5A/Nav1.5 were over-expressed in ovarian cancer cell lines SKOV-3 and ovarian cancer specimens at mRNA and protein levels. TTX 30 μmol/L inhibited the intracellular Na+ concentration by (41.51±0.41)%. TTX also suppressed the invasion and migration capacities of SKOV-3 cells by (33.80±1.6)% and (43.60±2.9)%, respectively. The difference was significant (P<0.05). Conclusion:SCN5A/Nav1.5 is involved in the metastasis progression of ovarian cancer in vitro and plays an important role in the initiation and progression of ovarian cancer. It may become a target for ovarian cancer therapy.
